Comparative study of the transfection efficiency of commonly used viral vectors in rhesus monkey (Macaca mulatta) brains
نویسندگان
چکیده
Viral vector transfection systems are among the simplest of biological agents with the ability to transfer genes into the central nervous system. In brain research, a series of powerful and novel gene editing technologies are based on these systems. Although many viral vectors are used in rodents, their full application has been limited in non-human primates. To identify viral vectors that can stably and effectively express exogenous genes within non-human primates, eleven commonly used recombinant adeno-associated viral and lentiviral vectors, each carrying a gene to express green or red fluorescence, were injected into the parietal cortex of four rhesus monkeys. The expression of fluorescent cells was used to quantify transfection efficiency. Histological results revealed that recombinant adeno-associated viral vectors, especially the serotype 2/9 coupled with the cytomegalovirus, human synapsin I, or Ca2+/calmodulin-dependent protein kinase II promoters, and lentiviral vector coupled with the human ubiquitin C promoter, induced higher expression of fluorescent cells, representing high transfection efficiency. This is the first comparison of transfection efficiencies of different viral vectors carrying different promoters and serotypes in non-human primates (NHPs). These results can be used as an aid to select optimal vectors to transfer exogenous genes into the central nervous system of non-human primates.
منابع مشابه
Diagnosis and surgical management of an intraocular foreign body secondary to ballistic wound in a Rhesus macaque (Macaca mulatta)
Background: Intraocular foreign bodies (IOFBs) such as air gun pellet is a rare finding in wild animals like Rhesus macaque (Macaca mulatta). The purpose of the present scientific report is to describe the surgical retrieval of IOFB secondary to ballistic wound in a wild Rhesus macaque. Case description: A juvenile female wild Rhesus macaque was brought with the history of swollen and inflamed ...
متن کاملInfluence of Naturally Occurring Simian Foamy Viruses (SFVs) on SIV Disease Progression in the Rhesus Macaque (Macaca mulatta) Model
We have investigated the influence of naturally occurring simian foamy viruses (SFVs) on simian immunodeficiency virus (SIV) infection and disease in Indian rhesus macaques. Animals were divided into two groups based upon presence or absence of SFV; in each group, eight monkeys were injected with SIV(mac239) virus obtained from a molecular clone and four were injected with medium. Blood was col...
متن کاملSalience of caller identity in rhesus monkey (Macaca mulatta) coos and screams: perceptual experiments with human (Homo sapiens) listeners.
Recent evidence from acoustic analysis and playback experiments indicates that adult female rhesus monkey (Macaca mulatta) coos are individually distinctive but their screams are not. In this study, the authors compared discrimination of individual identity in these sounds by naive human listeners who judged whether 2 sounds had been produced by the same monkey or 2 monkeys. Each of 3 experimen...
متن کاملJournal of Experimental Psychology: Animal Learning and Cognition Do You See What I See? A Comparative Investigation of the Delboeuf Illusion in Humans (Homo sapiens), Rhesus Monkeys (Macaca mulatta), and Capuchin Monkeys (Cebus apella)
متن کامل
Developmental stages in the rhesus monkey (Macaca mulatta).
Thank you very much for reading developmental stages in the rhesus monkey macaca mulatta. Maybe you have knowledge that, people have look hundreds times for their chosen books like this developmental stages in the rhesus monkey macaca mulatta, but end up in malicious downloads. Rather than reading a good book with a cup of coffee in the afternoon, instead they juggled with some harmful bugs ins...
متن کامل